what-is-the-purpose-of-soaking-the-gel-derived-from-the-initial-step-of-southern

Question

What is the purpose of soaking the gel derived from the initial step of Southern blotting in a denaturing buffer?

Accepted Answer

To convert the double-stranded DNA into a single-stranded DNA.

Answer

To identify the genes associated with a DNA fragment.

Answer

To deactivate DNA synthesis.

Answer

To degrade the unnecessary DNA fragments.