Which of the following tools of DNA technology is incorrectly paired with its use? a. electrophoresis—separation of DNA fragments b. DNA ligase—cutting DNA, creating sticky ends of restriction fragments c. DNA polymerase—polymerase chain reaction to amplify sections of DNA d. reverse transcriptase—production of cDNA from mRNA

Verified step by step guidance

1. Identify the function of each tool mentioned in the options: Electrophoresis is used for separating DNA fragments based on size; DNA ligase is used for joining DNA fragments together; DNA polymerase is used in PCR to amplify DNA; Reverse transcriptase is used to synthesize DNA from RNA.
2. Compare the stated uses with the known functions: Electrophoresis correctly matches its use; DNA polymerase correctly matches its use; Reverse transcriptase correctly matches its use.
3. Identify the mismatch: DNA ligase is stated to cut DNA and create sticky ends, but its actual function is to join DNA fragments together, not cut them.
4. Conclude that the incorrect pairing is: b. DNA ligase—cutting DNA, creating sticky ends of restriction fragments.
5. Correct the mismatch: DNA ligase should be paired with its use as an enzyme that joins DNA fragments together, often used in the cloning process to insert fragments into vectors.