18. Biotechnology
Steps to DNA Cloning
Multiple Choice
Multiple ChoiceWhich arrangement of the following four enzymes represents the order in which they would be used in a typical gene-cloning experiment resulting in the insertion of a cDNA into a bacterial plasmid? Begin with the gene's mRNA transcript.
A
Restriction enzyme, reverse transcriptase, DNA polymerase, DNA ligase
B
Restriction enzyme, DNA ligase, reverse transcriptase, DNA polymerase
C
Reverse transcriptase, DNA ligase, DNA polymerase, restriction enzyme
D
Reverse transcriptase, restriction enzyme, DNA polymerase, DNA ligase
E
Reverse transcriptase, DNA polymerase, restriction enzyme, DNA ligase
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