Which set of enzymes is involved in nucleotide excision repair?

• A. Nuclease, DNA polymerase, primase
• B. DNA polymerase, helicase, primase
• C. Ligase, nuclease, and primase
• D. Hydrolase, nuclease, and ligase
• E. nuclease, DNA polymerase, and ligase

Answer: (E)

Verified step by step guidance

1. Understand the process of nucleotide excision repair (NER), which is a mechanism to repair damaged DNA by removing a small section of the DNA strand containing the lesion and filling in the gap with newly synthesized DNA.
2. Identify the role of each enzyme involved in NER: Nuclease cuts out the damaged section of DNA, DNA polymerase synthesizes new DNA to fill the gap, and ligase seals the newly synthesized DNA into the existing strand.
3. Review the options provided and match the enzymes to their roles in NER. Nuclease is responsible for excising the damaged DNA, DNA polymerase fills in the gap with new nucleotides, and ligase connects the new DNA to the existing strand.
4. Eliminate options that include enzymes not involved in NER, such as primase, which is involved in the initiation of DNA synthesis, or helicase, which unwinds DNA strands during replication.
5. Confirm that the correct set of enzymes for nucleotide excision repair is nuclease, DNA polymerase, and ligase, as these are the enzymes that perform the necessary steps in the repair process.