In 1981, a stray black cat with unusual rounded, curled-back ears was adopted by a family in Lakewood, California. Suppose you owned the first curl cat and wanted to breed it to develop a true-breeding variety. Describe tests that would determine whether the curl gene is dominant or recessive and whether it is autosomal or sex-linked. Explain why you think your tests would be conclusive. Describe a test to determine that a cat is true-breeding.

Verified step by step guidance

1. First, determine if the curl gene is dominant or recessive by breeding the original curled-ear cat with a normal-eared cat. If any of the offspring have curled ears, the gene is likely dominant. If none do, then it may be recessive, and a second generation (F2) might need to be examined by breeding the F1 offspring together to see if the trait reappears.
2. Next, to test if the curl gene is autosomal or sex-linked, observe the distribution of the trait among male and female offspring. If the trait appears equally in both males and females, it is likely autosomal. If the trait appears predominantly in one sex, it may be sex-linked. This can be further tested by breeding males and females separately with normal-eared cats and observing the trait distribution in their offspring.
3. To confirm your findings from the initial tests, repeat the breeding experiments with multiple pairs to ensure consistent results across different genetic backgrounds and to rule out any environmental influences or anomalies in the initial breeding.
4. To determine if a cat is true-breeding for the curled-ear trait, breed two cats that both have curled ears. If all their offspring also have curled ears over several generations, it suggests that the parents are homozygous for the curl gene, indicating a true-breeding line.
5. Document all breeding experiments, offspring, and traits observed meticulously. This data will provide a solid foundation for drawing conclusions about the genetic nature of the curled-ear trait and will be invaluable for developing a true-breeding variety of curl cats.