Multiple Choice
Which of the following is NOT required for the reactions in dideoxy sequencing?
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Dideoxy Sequencing
Multiple Choice
Dideoxy sequencing is also known as chain termination sequencing because:
A
The dideoxy nucleotide prevents further synthesis of DNA due to the lack of a free 5' carbon.
B
The dideoxy nucleotide prevents further synthesis of DNA due to the lack of a free 3' OH.
C
The dideoxy nucleotide prevents further synthesis of DNA due to the lack of a nitrogen-containing base.
D
Chain termination is the same as sequencing by synthesis.
E
None of the above are correct.
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